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Fig. 6. LIN-39 and CEH-20 bind cooperatively to two consensus Hox/PBC binding sites
in vitro. The bottom arrow indicates bands corresponding to probes bound to
LIN-39 alone and the top arrow indicates bands corresponding to probes bound
to LIN-39/CEH-20 heterodimers. In the first four lanes, an oligonucleotide
(Antp/Exd) containing a binding site for a Drosophila Hox protein,
Antennapedia, and its co-factor, Extradenticle, is used as a positive control.
LIN-39 and CEH-20 bind the wild-type site 1 (S1) and site 2 (S2) efficiently,
but not the mutated sites (S1M and S2M). Site 2 shows greater binding affinity
for LIN-39/CEH-20 heterodimers than does site 1, and LIN-39 alone can bind
site 2 but not site 1.
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