Fig. 1. Insertion of the P-element, which causes the Abd-B^lac1 mutation, and pattern of expression of the same P-element inserted outside the BX-C. (A) Scheme of the Abd-B^lac1 insertion, showing the P-element and the place of integration in the Abd-B DNA. The Abd-B 3' regulatory DNA included in the P-element is marked in white. The oval and the square within this region represent the Fab-8 boundary and 3' iab-8 regulatory sequences, respectively. The Abd-B promoter region (Abd-Bpp) included in the same transposon is marked in gray, the lacZ gene in blue and the rosy gene in orange. The arrow in the genomic DNA represents the transcription start site of the Abd-B m RNA. iab-5 to iab-8 and Fab-8 endogenous sequences are represented to the left of the insertion. The numbers refer to the coordinates in kb (Martin et al., 1995). Below the line representing the genomic DNA, we show the Abd-B transcription unit, indicating the Abd-B m (class A) and the Abd-B r (class B, class C) transcripts. There is also an Abd-B transcript, expressed in PS15 (Kuziora and McGinnis, 1988), not represented in the figure. B, BamHI; R, EcoRI; H, HindIII; S, SalI. (B,C) Expression of the lacZ gene when the P-element is inserted outside the BX-C in a stage 11 (B) and a stage 14 embryo (C).

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