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Fig. 4. HpTb protein is accumulated into PMC nucleus after blastula stage. (A)
Developmental western blot. Thirty micrograms of protein prepared from embryos
at various developmental stages were run in parallel. Detection procedure of
HpTb protein is described in text. U, unfertilized egg; 16, 16-cell stage; M,
morula; UB, unhatched blastula; HB, hatched blastula; MB, mesenchyme blastula;
G, gastrula; Pr, prism; Pl, pluteus. (B,C) Immunostaining of embryos with
antibodies to HpTb. (B) Morula; (C) mesenchyme blastula. Scale bar: 50
µm.
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