Fig. 5. Effect of injection of HpTb morpholino antisense oligonucleotides on the embryogenesis. (A,C,E,G) Control embryos injected with lacZ morpholino (7x10⁸ molecules/egg). (B) Embryo injected with low amounts of HpTb morpholino (1x10⁸ molecules/egg). (D,F,H-J) Embryos injected with HpTb morpholino (7x10⁸ molecules/egg). The repression of translation of HpTb was confirmed using indirect immunofluorescence with anti-HpTb antibodies on mesenchyme blastula, (I) bright-field view, (J) epifluorescence view. (K) Embryo co-injected with 1.5 pg of HpTb-mRNA and HpTb morpholino (7x10⁸ molecules/egg). Embryos at 28 hours (C,D,I,J), 40 hours (E,F), 48 hours (A,B,K) and 72 hours (G,H). Scale bar: 50 µm.

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