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Fig. 9. Loss of organizing activity of micromeres by injecting with HpTb morpholino. (A,B) Chimera composed of animal cap mesomeres from normal embryo with the micromere quartet isolated from a 16-cell stage embryo developed from zygotes injected with 7x108 molecules of HpTb morpholino (A, vegetal view; B, lateral view). (C) Control embryo. (D,E) The micromeres were labelled with rhodamine-dextran. Chimera composed of micromere quartet from normal embryo with the animal cap mesomeres isolated from a 16-cell stage embryo developed from zygotes injected with 7x108 molecules of HpTb morpholino (D, lateral view; E, vegetal view). The animal cap mesomeres were labelled with rhodamine-dextran. Left, bright-field views; right, epifluorescence views. Scale bar: 50 µm.





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