Fig. 6. Phox2b acts as an activator in promoting neurogenesis and inducing Islet1, Nkx6.1 and endogenous Phox2b. (A-B') at 48 h.a.e. of PHDVP16 (A) but not of PHDEnR (B), the transfected cells have emigrated to the mantle layer as shown by GFP fluorescence. Anti-Islet1/2⁺ cells are found in ectopic location after expressing the PHDVP16 (arrowheads, A') but not the PHDEnR construct (B'). (C-J') in situ hybridization with Ngn2 (C' ,E' ,F'), Ngn1 (D'), Phox2b (G' ,J') probes at 6 h.a.e. of PHDVP16 (C'), PHDEnR (E') and SHDVP16 (F'), at 20 h.a.e. of PHDVP16 (D'), or at 24 h.a.e. of PHDVP16 (G' ,H') or PHDEnR (I' ,J'). GFP fluorescence (A-C) or in situ hybridization with a GFP probe (D-J) shows the extent of electroporation. At 6 h.a.e. of PHDVP16, Ngn2 is upregulated in the transfected area (C') while PHDEnR or SHDVP16 constructs have no effect (E' ,F'). Forced expression of PHDVP16 also promotes expression of Ngn1 (D'), induces the endogenous Phox2b gene (G') and expands Nkx6.1 expression (H'), while PHDEnR does not induce Phox2b (I') and represses Nkx6.1 (J').

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