Fig. 1. unc-62 molecular cloning, transcript structure and sequence comparisons. (A) Genetic and physical maps of the unc-62 region on LGV. Genetic mapping placed unc-62 on the left arm of LGV, between two strain-specific polymorphisms stP3 and RW#L63 and in the region uncovered by deficiency sDf27 but not sDf50. Cosmids mapped to this region by the C. elegans genome consortium are shown below. unc-62 putative regulatory sequences and coding sequence reside on cosmids T08H10 and T28F12, respectively; both the regulatory and coding sequences are included in the fosmid clone H06N16. (B) Structure of unc-62 transcripts and locations of mutant lesions. unc-62 produces four alternatively spliced transcripts that differ in the choice of the first exon (1a or 1b) and the seventh exon, which is the first exon of the homeobox region (7a or 7b). The HM domain is encoded by exons 2, 3, 4 and 5, while the TALE homeodomain is encoded by exons 7a or 7b, 8 and 9. Locations of the point mutations t2012 and e644, the deletions s472 and ct344, and the left inversion breakpoint of e917 are indicated (see Fig. 6 for a more precise map of the rearrangements). (C,D) Sequence similarities of the conserved UNC-62, Homothorax, and murine Meis1 HM and homeodomains, respectively. Dashes indicate identical residues.

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