Fig. 1. Normal expression patterns of Fgf8, Lmx1b and Wnt1. In situ hybridization of serial sections of the same embryos at stage 10 (A,C,E) and stage 12 (B,D,F), for Fgf8 (A,B), Lmx1b (C,D) and Wnt1 (E,F). At stage 10, Lmx1b and Wnt1 are expressed in the mesencephalon and metencephalon. Their expression overlaps with Fgf8 expression in the metencephalon. At stage 12, Lmx1b is expressed strongly in the mesencephalon, but in the metencephalon its expression is weak. Wnt1 expression in the metencephalon has almost disappeared. (G,H) Whole-mount in situ hybridization for Lmx1b (blue) and Fgf8 (red) (G), and for Wnt1 (blue) and Fgf8 (red) (H). Both Lmx1b and Wnt1 are expressed next to Fgf8 expression at the mes-metencephalic boundary. Scale bars: 250 µm. mes, mesencephalon; met, metencephalon.

Return to article