Fig. 5. Regulation of Fgf8 by Lmx1b. (A-C) Cell-autonomous repression of Fgf8 by Lmx1b. Whole-mount in situ hybridization for Fgf8 (blue) at 12 hours after electroporation (A, control; B, experimental side). Section of the same embryo stained for Fgf8 (blue) and Lmx1b (red) (C). Note that Fgf8 expression was repressed in the Lmx1b-expressing cells (C). (D-F) Non cell-autonomous induction of Fgf8 by Lmx1b. Whole-mount in situ hybridization for Fgf8 (blue) at 24 hours after electroporation (D, control; E, experimental side). Section of the same specimen stained for Fgf8 (blue) and Lmx1b (red) (F). Fgf8 is still repressed in Lmx1b-misexpressing cells (asterisks indicated by red arrows, E), but around the Lmx1b-expressing cells (asterisks), Fgf8 is induced ectopically in the caudal metencephalon (black arrows in E). Scale bars: 100 µm. Views from the control side are printed in reverse for the comparison with the experimental side throughout the paper.

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