Fig. 7. Effects of Lmx1b and Wnt1 misexpression on isthmus-related genes. (A-E) Wnt1 induction by Lmx1b misexpression. In situ hybridization for Wnt1 (blue; A,B,D) and Lmx1b (red; C,E) on the same embryo 24 hours after electroporation. (A) View from the control side; (B,C) view from the experimental side. (D,E) High-power magnifications of boxed areas in B,C, respectively. Wnt1 is expressed in the Lmx1b-expressing cells (D,E). (F-Q) Effects of Wnt1 misexpression on Fgf8, Otx2, Gbx2 and Lmx1b. In situ hybridization for Fgf8 (F,G), Lmx1b (I,J), Otx2 (L,M) and Gbx2 (O,P). (F,I,L,O) View from the control sides. (G,J,M,P) View from the experimental side. (H,K,N,Q) Fluorescence micrograph of GFP to show transfection efficiency. Fgf8 expression expanded caudally by Wnt1 misexpression (G). Lmx1b, Otx2 and Gbx2 expression is not affected (J,M,P). Scale bars: 500 µm in H,K,N,Q; 200 µm in C; 50 µm in E.

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