Fig. 5. Gas1^-/- limbs have altered programmed cell death (PCD) patterns. PCD during limb development was assessed by TUNEL fluorescent labeling. In Gas1^-/- (-/-), the TUNEL assay shows reduced and delayed PCD relative to control (con) at E11.5 (A,A'), E13.5 (B,B') and E14.0 (C,C'). White solid arrows indicate cell death at the opaque patch; white open arrows, the anterior margin; open arrowheads, the interdigital mesenchyme; white arrowheads, the forming joints. The limbs were sectioned horizontally and the digits are as labeled (I-V).

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