Fig. 6. Molecular analysis of Gas1^-/- limbs for patterning genes. Control (con; A-E) and Gas1 mutant (-/-; A'-E') forelimbs were compared for expression of Shh, gremlin, Bmp2, Bmp4, and Ptc1 by ISH at E10.5. (A,A') Shh expression appears normal in Gas1^-/- limbs. (C,C') Bmp2, (D,D') Bmp4, and (E,E') Ptc1 expression is not obviously altered. (B,B') gremlin expression appears to be slightly reduced in the limb mesenchyme in the mutant. Hoxd13 expression was examined at E11.5 (F,F') and found to be normal in the mutant — in a smaller domain proportional to the smaller size of the limb. Expression of Ptc1 (dorsal view) and Hoxd13 (lateral view, arrowheads indicate the forelimb expression) was assessed by whole-mount ISH; the others, by ³⁵S-ISH on horizontal sections with anterior at top.

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