Fig. 7. ISH of Fgf expression in the Gas1^-/- AER. (A-C) Fgf4, Fgf9 and Fgf17 gene expression (arrows) is normal in Gas1^-/- limb at E10.5 as determined by ³⁵S-ISH on horizontal sections; anterior at top. Control (con; D-G) and Gas-1^-/- (-/-; D'-G') collected at E9.5 (D,D'), E10.0 (E,E'), E10.5 (F,F') and E11.5 (G,G') were analyzed for Fgf8 expression by whole-mount ISH. Fgf8 expression is initiated but not maintained. Black arrowheads indicate the location of the AER; white arrowheads, Fgf8 expression in other regions as an internal control (in the tailbud in E and E' and branchial arches in F' and G'); open arrowheads, reappearance of small patches of Fgf8 expression in the posterior AER (G'); flb, forelimb; hlb, hindlimb.

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