Fig. 3. Ptc2 and Gli1 transcripts are polarized in ameloblasts. In situ hybridization on control incisors at 1 dpp at the level of presecretory (A,D) and young secretory ameloblasts (B,C,E,F). Oblique sections (A,D). Frontal sections (B,C,E,F). The dotted lines indicate the ameloblast apical plasma membrane. Ptc2 (A,C) and Gli1 (B) mRNAs are enriched at the basal and perinuclear compartment (green arrow) of ameloblasts, causing the signal (red) to mask the blue staining of ameloblast nuclei, whereas the apical pole (red arrow) displays considerably less signal. The extent of the stratum intermedium layer (si) is indicated by bars (A,D). In contrast, mRNAs for Msx2 (D), Ptc1 (E) and Dlx3 (F) are enriched apically (red arrow). Scale bars: 100 µm.

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