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Fig. 4. Analysis of Shh expression and Shh responsiveness at the cap stage in Smo mutants. Schematic drawings showing tissue organization of molars at the cap stage and Shh expression (stippled) and responsiveness (yellow) within the dental epithelium and mesenchyme (A,B). The green arrows show movement of Shh protein. Alterations of Shh responsiveness in the dental epithelium and its preservation in the mesenchyme in Smo mutants are summarized in B. In situ hybridizations on frontal sections of molars for Shh (C,D); Ptc1 (E,F); Gli1 (G,H); Ptc2 (I,J) and Hip1 (K,L). DM, dental mesenchyme; DS, dental sac; EK, enamel knot; IDE, inner dental epithelium; ODE, outer dental epithelium; SR, stellate reticulum. Scale bars: 100 µm.





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