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Fig. 3. Flat-mount in situ hybridization of E7.5 chick retinas electroporated with EnRaxL{Delta}C (A-F), EnRax{Delta}C (G-L), EnRaxLHD (M,N), and EnIrx (O,P) viral constructs. Endogenous visinin (A-C,G-I,M-P) and Rxrg transcripts (D-F,J-L) are represented by the purple stain and exogenous EnRax{Delta}C, EnRaxL{Delta}C, EnRaxLHD and EnIrx by the green-blue stain. The sections of the retina samples in B,E,H,K are shown in C,F,I,L, respectively. Scale bars: in A, 2 mm for all the flat-mount images; in C, 30 µm for C,F,I,L.





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