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Fig. 5. CAT activities were assayed in COS cells transiently transfected with the
RET1-CAT reporter construct (A) together with Rax or RaxL expression vectors
(B) or with combinations of different ratios of expression vectors as
indicated (C). The CAT activity from each transfection was normalized for
internal transfection efficiency (see Materials and Methods). The relative CAT
activity was normalized to the value from empty vector (B) or from RaxL
transfection alone (C). The data presents the average value of triplicates.
EnRax* represents EnRax
C, EnRaxL
C or EnRaxLHD
indicated in C.