Fig. 1. Egg chamber development in wild-type and mutant ovaries. (A) A Drosophila ovariole showing the germarium and egg chambers from stages 3-10 (anterior towards the left). Each egg chamber contains 16 germ cells, including 15 nurse cells (nc) and one oocyte (o). Polar cells are shown in red. Stalk cells separate adjacent egg chambers. Border cells are shown in blue. (B-D) Normarski optics images of egg chambers from enhancer trap line PZ6356, stained for ß-galactosidase, which is expressed at highest levels in the border cells (arrows) and the oocyte nucleus (arrowheads). (B) A wild-type, stage 10 egg chamber with one cluster of border cells (BCs) at the nurse cell/oocyte border (ON, oocyte nucleus). (C) A stage 10 mosaic egg chamber containing 54C2 mutant cells and three clusters of border cells. (D) A stage 10 mosaic egg chamber containing cos2 mutant cells and two clusters of border cells. (E-G) Fluorescence micrographs of a border cell cluster double stained with anti-FAS3 (green) and rhodamine phalloidin (red). In wild-type egg (E), one pair of anterior polar cells stains. The polar cells (pc) are surrounded by border cells (bc). In both 54C2 (F) and cos 2 (G) mosaic egg chambers, extra border cells surround extra polar cells. (H-J) Normarski optics images of egg chambers stained for ß-galactosidase from enhancer trap line A101 (neu-lacZ), which is a marker for mature polar cells. (H) In wild-type, polar cells are located at the anterior and posterior poles. In egg chambers containing 54C2 (I) or ptc (J) mosaic chambers, polar cells are found at many locations. (K-P) Fluorescence micrographs of egg chambers stained with anti-FAS3 (K,L,N,O) or DAPI (M,P). (K) A series of wild-type egg chambers up to stage 5. (L) A wild-type, stage 9 egg chamber. In the germarium and stage 1 egg chamber, all follicle cells are FAS3 positive. Polar cells are indicated by white arrowheads. (M) A wild-type, stage 9 egg chamber stained with DAPI. (N-P) Egg chambers from eya^3cs/eya^E(P)10 females. Egg chambers are either arrested early in oogenesis (N) or are covered with FAS3-positive cells (O). In the egg chamber in P, which is the same one as in O, the germ cells are degenerating. Scale bars: in B, 50 µm for B-D; in E, 10 µm for E-G; in H, 50 µm for H-J; in K and N, 25 µm for K-M and N-P, respectively.

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