Fig. 4. The relationship between EYA and HH pathway genes. (A-C) Fluorescence confocal micrographs of a Pka mosaic egg chamber. GFP expression is shown in green. Both EYA (nuclear) and FAS3 (membrane) are shown in red. The Pka mutant cells lack GFP and are outlined by broken lines in A-C. (B) A composite image of a z-series of adjacent confocal sections showing the expression of EYA and FAS3 to demonstrate that some FAS3-positive cells have EYA expression, whereas others do not. The YZ plane corresponding to a slice through the Z series of the clone shown in B is shown in (B'). Of the FAS3-positive cells which have red membrane staining in (B), two cells (^*) are EYA positive and four cells (arrows) are EYA negative and lack nuclear staining. (C) Merged picture of GFP, eya and FAS3 in a single section from A. (D-F) Fluorescence micrographs of a Pka mosaic egg chamber stained for EYA (green) and ß-galactosidase (red) from the enhancer trap line A101. Scale bars: in A, 5 µm for B' and 10 µm for A-C; in D, 10 µm for D-F.

Return to article