Fig. 5. The relationship between ci and eya. (A) Fluorescence micrographs of a ci mosaic egg chamber showing GFP expression in green and EYA expression in red. Cells that lack GFP are ci mutant cells. Co-localization of GFP and EYA appears yellow. (B,C) Fluorescence images of egg chambers double stained for FAS3 (green) and ptc-lacZ (red). (B) A wild-type egg chamber in which anterior polar cells (arrow) stain. (C) An eya mosaic egg chamber with two pairs of anterior polar cells (arrows), both of which have ptc-lacZ expression. (D) Fluorescence images of an egg chamber expressing the constitutively active form of CI (CI^AC) (see Materials and Methods). Cells with ß-galactosidase expression (green) express CI^AC. Expression of EYA is shown in red. (E,F) Fluorescence images of a Pka mosaic egg chamber double stained for CI (gray in E, red in F) and GFP in green. (G) Fluorescence micrographs of an eya mosaic egg chamber double stained for EYA (red) and CIN (green), which recognizes both CI^AC and CI^R (eya mutant clones are outlined) (H,I) Fluorescence images of an eya mosaic egg chamber double stained for CI (gray in H, red in I) and GFP in green. Scale bars: in A, 10 µm for A,D,G; in B, 25 µm for B,C; in E, 10 µm for E,F,H,I.

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