Fig. 6. The phenotype of overexpression of eya in the ovary. Egg chambers stained with DAPI (A,C,C',E) to label nuclei or with anti-FAS3 (B,D,F,G) to label the polar cells. (A) A compound egg chamber produced by overexpression of eya in the ovary. (B) The same egg chamber as in A, which has four pairs of polar cells (arrows). (C) Compound egg chambers that never leave the germarium. (C') A wild-type germarium and the first two egg chambers for comparison. (D) A stage 8 egg chamber in which posterior polar cells express FAS3. Anterior polar cells appear to be missing. (E) A fused egg chamber. (F,G) Two different focal planes of the same egg chamber as in E. There are three pairs of polar cells (arrows) in this compound egg chamber. (H) A stage 10 egg chamber in which border cells express GFP and overexpress EYA. Migration is delayed. Arrowheads indicate the centripetal follicle cells and the normal extent of border cell migration at this stage. Scale bars: in A, 50 µm for A,B; in C, 25 µm for C,D; in C', 25 µm for C'; in E, 50 µm for E-G; in H, 50 µm for H.

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