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Fig. 10. RT-PCR molecular marker analysis on animal caps injected with various combinations of BMP, Wnt and Nodal inhibitors, as indicated, and grown to stage 30/31. Doses were as follows: (1) 200 pg Smad7; (2) 25 pg chd; (3) 25 pg chd+200 pg Nxfz8; (4) 25 pg chd+500 pg cer-S; (5) 25 pg chd+1000 pg cer-S; (6) 25 pg chd+200 pg Nxfz8+500 pg cer-S; (7) 25 pg chd+500 pg cer{Delta}C1; (8) 500 pg cer{Delta}C1; (9) 660 pg chd.





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