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Fig. 2. Specification assays on dorsal ectoderm (DE) of stage 10.5 embryos. DE was explanted as outlined in the scheme, grown to stage 12.5. (J), to stage 22/23 (A-I) or to stage 30/31 (K-M), and finally processed for in situ hybridization with probes for nrp1, Xotx2, XBF-1, Xrx1, Xvax1b, Xemx1, Xkrox20, XhoxB9, cardiac actin and chd as indicated.





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