Fig. 4. Tissue recombination induction assays. Conjugates were made by recombining stage 9 animal caps with the involuted anterior dorsal mesendoderm (ADME, green) of stage 10.5 embryo (A-E, upper scheme on the left) or with the presumptive notochordal fragment (brown) of stage 11 embryo (F-J, lower scheme on the left). Conjugates were grown to stage 22/23 and assayed by in situ hybridization for expression of the neural markers nrp1 (A,F), Xotx2 (B,G), Xrx1 (C,H), XBF-1 (D,I), Xemx1 (E,J). (K) RT-PCR analysis of the expression of neural markers in similar conjugates: AC, animal caps; WE, whole embryo; `green' and `brown' correspond to the colored fragments in the schemes.

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