Fig. 3. Sequence analysis. (A) Multiple sequence alignment of the most conserved domain of runt-like proteins including Tu-run. Dr_Runt1, Danio rerio runt; Aml1, human AML1 protein; Sp_runt, Strongylocentrotus purpuratus; Cs_runt 1 and 2, Cupiennius salei runt1 and 2; Lozenge, Drosophila melanogaster loz; Dm_runt Drosophila melanogaster runt. (B) Multiple alignment of the C-terminal VWRPY motif of Runt-like proteins. He_Runt1, Heliocidaris erythrogramma; Gg_runtb, chick runtB; Xl-aml1, Xenopus laevis AML-like protein 1; Mm_runt1 and 3, mouse runt 1 and 3; Hs-aml1 and 2a, human AML-like proteins 1 and 2a; Dr_runtb, Danio rerio runt b; Dm_loz, Drosophila melanogaster lozenge; Ds_runt, Drosophila simulans runt; Ce_runt1, Caenorhabditis elegans runt1. (C) Unrooted maximum likelihood cladogram of the multiple alignment shown in A. (D) Amino acid sequence alignment of the cloned region of Tu-pax3/7 with other PgIII genes. Boxed region shows the amino acids used for the phylogenetic analysis shown in F. Sa_PB1 and 2, Schistocerca americana Pairberry 1 and 2; Dm_prd, Dm_gbp-D, Dm_gbp-P, Drosophila melanogaster paired, gooseberry paired distal and gooseberry paired proximal respectively; pax 7 and 3, mouse pax7 and 3. (E) Unrooted maximum likelihood cladogram of the region of the alignment shown in D including representatives of all pax group genes. Pax1-9, mouse Pax proteins; pox m, pox messo; spark, sparkling; ey, eyeless proteins from Drosophila melanogaster; paxD and paxb from Acropora millepora. (F) Unrooted maximum likelihood cladogram of the boxed region of the alignment in D. Tu-run GenBank accession number, AY148194; Tu-pax3/7 GenBank accession number: AY148194.

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