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Fig. 5. Expression of Tu-pax3/7 in blastoderm stage embryos. (A,C,E,G,I) Surface view of blastoderm stage embryos hybridised for Tu-pax3/7 RNA. (B,D,F,H,J) Deeper focal plane of blastoderm stage embryos hybridised for Tu-pax3/7 RNA. (A,B) Tu-pax3/7 RNA is first expressed in three stripes of cells across the ventral surface, corresponding to the pedipalp segment, the second walking leg and the fourth walking leg. (C,D) Expression of Tu-pax3/7 RNA becomes apparent in a stripe in the first walking leg segment (asterisks). (E,F) Embryo with four detectable stripes of Tu-pax3/7-expressing cells, corresponding to the pedipalps, the first and second walking legs and the fourth walking leg. (G,H) Expression of Tu-pax3/7 RNA becomes apparent in a stripe in the third walking leg segment (asterisks). (I,J) Embryo with five detectable stripes of Tu-pax3/7 expression, corresponding to all the segments of the prosoma except the chelicera segment. A small stripe is also forming posterior to the fourth walking leg segment (arrows). Tu-pax3/7 expression is starting to disappear from cells in the centre of each stripe in the prosoma. Scale bars: 50 µm. P, pedipalp; 1-4, walking legs. Anterior to the left, dorsal up.





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