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Fig. 6. Expression of Tu-pax3/7 and Distalless. (A-D) Lateral view of
embryos hybridised for Tu-pax3/7 RNA (blue). Anterior to the left,
dorsal is up. (A) Surface view of an embryo showing the full pattern of
Tu-pax3/7 RNA stripes in the prosoma. (B) Deeper focal plane of an
embryo showing one stripe of cells expressing Tu-pax3/7 RNA in the
opisthosoma, as well as the prosoma pattern. (C) Later epidermal expression of
Tu-pax3/7. Cells expressing Tu-pax3/7 RNA are visible in all
the prosoma segments and in three segments in the opisthosoma. (D) Late
expression of Tu-pax3/7 in the nervous system. (E-H) Ventral view of
embryos hybridised for Tu-pax3/7 RNA (blue) and stained for Dll
protein (brown). Anterior to the left. (E) Cells within the anterior region of
the Tu-pax3/7 RNA-expressing stripes in the prosoma begin to express
Dll protein. In cells expressing Dll protein, expression of Tu-pax3/7
RNA rapidly becomes undetectable. (F) Dll expression spreads across the entire
Tu-pax3/7 expression domain. Tu-pax3/7 becomes restricted to
a square group of cells just ventral to the Dll-expressing limb bud. (G) Dll
expression retreats from the proximal regions of the limb bud. (H) Expression
of Tu-pax3/7 in the nervous system. The arrow indicates the primordia
of the fourth walking leg. (I-L) Lateral view of embryos hybridised for
Tu-pax3/7 RNA (blue) and stained for Dll protein (brown). Anterior to
the left, dorsal up. (I) Expression of Dll protein appears in
Tu-pax3/7-expressing cells in each stripe. These cells rapidly lose
expression of Tu-pax3/7. (J) Expression of Dll and loss of
Tu-pax3/7 expression spreads across the stripe. (K) Dll expression
retreats from proximal regions of the limb. (L) Expression of
Tu-pax3/7 in the nervous system. Dark staining in the anterior of the
embryo is caused by damage during preparation. (M-P) Embryos oriented to
examine expression of Tu-pax3/7 RNA (blue) and Dll protein (brown) in
the opisthosoma. Anterior parts of the germ band curl away from the focal
plane to the left; embryos are viewed from the ventral surface. (M) One broad
stripe of Tu-pax3/7-expressing cells is visible posterior to the
fourth walking leg stripe (marked by expression of Dll). This is joined by a
second stripe (N), and a third (O). Later, expression of Tu-pax3/7
RNA is visible in three stripes in the central nervous system (P). Arrowheads
indicate the first walking leg segment. Scale bars: 50 µm.
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