Fig. 1. Structure and phylogenetic analysis of HrzicN. (A) The putative amino acid sequence of HrzicN. Zinc finger motifs are italicized. (B) Molecular phylogenetic tree of Zic genes constructed using the amino acid sequences of the zinc finger motifs by the Neighbor-Joining method and the computer program, ClustalW. Numbers at the nodes represent bootstrap values. macho-1 is another Halocynthia Zic gene isolated as a muscle determinant. Cszicr1a, Cszicr1b and Cszicr2 are Zic genes of Ciona savignyi, another ascidian species. opa (odd-paired) is a Zic gene of Drosophila. Amphizic is an amphioxus Zic gene registered on the database. xGli1, hGli2, xGli3, hGli3 and xGli4 are members of vertebrate Gli family of zinc finger protein. Other genes given are vertebrate Zic family genes. DDBJ/GenBank accession numbers: AB092643(HrZicN), BAB19958(macho-1), AB057747(Cszicrla), AB057748(Cszicr1b), AB057740(Cszicr2), NP524228(opa), AJ252245(Amphizic), Q91690(xGli1), BAA25668(hGli2), Q91660(xGli3), CAB59315(hGli3), Q91661(xGli4), NM_009573(mZic1), NM_003412(hZic1), AB009564(xZic1), NM_009574(mZic2), NM_007129(hZic2), AB009565(xZic2), NM_131558(zZic2), NM_009575(mZic3), NM_003413(hZic3), AB005292(xZic3), NM_009576(mZic4), AK006747(mZic5), AF378304(hZic5), AB034983(xZic5).

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