Fig. 4. Expression of epidermal and neuroectodermal markers in HrzicN knockdown embryos at the early tailbud equivalent stage. (A-C,G-I) Embryos injected with HrzicNMO. (D-F,J-L) Control uninjected embryos. Expression of HrETR-1 (A,D), HrTBB2 (B,E), HrTRP (C,F), Hroth (G,J), HrEpiD (H,K) and HrzicN (I,L) was shown. Arrowheads in D-F,J indicate gene expression in the neural tube, which is lost or severely reduced in HrzicN knockdown embryos. Arrowhead in H indicates putative neural tube precursor cells without HrEpiD expression. Arrows in A,B,D,E indicate gene expression in the adhesive organ, which is unaffected in HrzicN knockdown embryos. (A-E,G-J) A dorsal view. (F,K,L) A lateral view.

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