Fig. 5. Detection of Easter catalytic domain in ea^D embryo extracts. Extracts were prepared from 0-4 hour embryos laid by wild-type, eaN/+, ea⁸/ea^5022rx1, ea^83l/ea^5022rx1 and ea^5.13/ea^5022rx1 females. Protein samples were separated on a 10% SDS polyacrylamide gel. The immunoblot was probed with anti-Easter antibodies. The Easter zymogen, the Easter catalytic domain (Ea CD), and a higher M_r form (Ea-X) are indicated by arrows. In this particular blot, the Easter zymogen co-migrates with a prominent cross-reacting 45 kDa band. In the ea^D alleles, an increased level of the Easter catalytic domain and a corresponding decrease in Ea-X level is observed.

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