Fig. 2. Quantitative analysis on the distribution of TH-positive sympathetic neurons. The average number of TH-positive neurons per section was calculated from around 20 serial frontal sections that were made at the cervical level of embryos at E12.5. Open and filled columns indicate the number of TH-positive neurons located at the original sites of the sympathetic ganglia and ectopic positions, respectively. (A) neuropilin 1 mutant embryos. 100%, 99.4% and 51.2% of TH-positive neurons are located at the original sites of the sympathetic ganglia in the wild-type embryo (1 embryo), heterozygous mutant embryos (2 embryos) and homozygous mutant embryos (3 embryos), respectively. (B) The Sema3A mutant embryos. 97.6% and 35.9% of TH-positive neurons are located at the original sites of the sympathetic ganglia in the wild-type embryos (3 embryos) and homozygous mutant embryos (2 embryos), respectively.
