Fig. 2. Double labeling of Olig2 and Nkx-2.2 from E4 to E12. Spinal cord sections from E4 (A), E5 (B), E6 (C), E7 (D), E8 (E,I), E9 (F,J,K), E10 (G,L) and E12 (H) were subjected to Nkx-2.2 immunohistochemical staining (in brown) followed by in situ hybridization (in blue) with Olig2 riboprobe. Only the ventral half of spinal cords are shown. In A, anti-Nkx2.2 immunostaining and Olig2 in situ hybridization were performed on immediately adjacent slides, and half of the ventral cord is aligned closely for comparison. In B, the overlapping region of the Olig2 domain and Nkx2.2 domain is indicated. The Olig2+/Nkx2.2+ cells and Olig2–/Nkx2.2+ cells are represented by arrows and arrowheads, respectively. I is a higher power view of F. J,K are higher power views of F. L is a higher power view of G.
