Fig. 8. Expression of Pdgfra, Olig2 and Nkx2.2 in the PDGFA mutants at P0 and P7. (A-H) Immediately adjacent sections from P0 wild-type (A-D) and mutant (E-H) spinal cords were probed with Pdgfra (A,E), Olig2 (B,F), Nkx2.2 (C,G) and MBP (D,H) by in situ hybridization. (I-P) Adjacent sections from P7 wild-type (I-L) and mutant (M-P) spinal cords were stained with Pdgfra (I,M), Olig2 (J,N), Nkx2.2 (K,O) and MBP (L,P). At these two stages, expression of Pdgfra and Olig2 is delayed and reduced in the mutants, whereas expression of Nkx2.2 and MBP is mostly affected in the white matter. Note the similar patterns of expression of Nkx2.2 and MBP, especially in the gray matter, at P0 (C-D,G-H).
