
Fig. 4. Proliferation of follicle cells is not extended in hop mutants. In wild-type (A) ovarioles, follicle cells cease proliferation after stage 6, as indicated by the lack of any PH3 staining cells (in green, with Fas III in red, DAPI in blue) in post stage 6 chambers. As in wild type, in hopmsv/hopGA32 (B) and in hopmsv/hopM38 (C) ovarioles, no PH3 staining can be seen after stage 6. This restriction is visible even within fused chambers where cysts of different maturity are distinct (arrowhead). (D) In hopmsv/hopM75 ovarioles marked with PZ80, chambers with fusions of two consecutive cysts have islands of ectopic polar cells at the intersection of the fused cysts. The number of polar cells in those islands is represented in the graph.