Fig. 5. TGFß2 mRNA and protein expression is elevated in the Rxra–/– embryo. (A) RNase protection assays showed that message levels for TGFß2 were elevated in the mutant (KO). WT, wild type. (B) Message levels for TGFß1 were not altered while MLC2a was elevated in the mutant. EF1
was used as a loading control. (C) Immunohistochemical analysis on transverse serial sections of E13.5 OFT regions demonstrated that TGFß2 protein was also elevated in the mutant. (D) E12.5 OFT also shows elevated TGFß2 in the mutant heart.
