Fig. 5. RBF expression blocks Tkv^Q253D induced proliferation. (Left panels) Confocal images of Flip-out/Gal4 lateral clones of the indicated genotype visualized by GFP signal. (Right panel) FACS analysis of a similar timing experiment. Black traces show the GFP^– internal control population, and gray filled traces show the GFP⁺ experimental population. Numbers in the top right-hand corner in the size histograms show the ratio of the mean FSC of GFP⁺ cells (experimental population) versus the mean FSC of GFP^– cells (control population). Clones expressing RBF and Tkv^QD contain as few cells as RBF-expressing clones (arrows). These cells do not show the characteristic cell cycle profile of Tkv^QD-expressing cells (DNA content histogram) and are bigger than cells expressing RBF alone (size histogram). Scale bar: 50 µm.

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