Fig. 5. rasp is required in posterior cells to activate Hh target gene expression in anterior cells. (A-L) Analysis of rasp^7F21 (rasp^–) clones in wing imaginal discs. Clones were generated in a M^–/+ background and marked by the absence of GFP shown in green. (B,E,H,K) Brackets highlight the domain of Hh-dependent gene expression in anterior cells. (C,F,I,L) The broken line marks the approximate position of the AP boundary. (A) A large anterior rasp^– clone is marked by the absence of GFP. This clone defines the position of the AP boundary from the anterior side. (B) The pattern of Ptc protein is shown in red and is indistinguishable from wild type. (C) Overlay of A and B. (D) A large posterior rasp^– clone is marked by the absence of GFP. This clone defines the position of the AP boundary from the posterior side. (E) The elevated domain of anterior Ptc protein is reduced in this disc (compare with B). (F) Overlay of D and E. (G) A large anterior rasp^– clone is marked by the absence of GFP. This clone defines the position of the AP boundary from the anterior side. (H) The domain of both anterior and posterior En protein is shown in red and is indistinguishable from wild type. The brackets mark the approximate position of anterior cells expressing En protein. (I) Overlay of G and H. The domain of anterior En protein is clearly detectable in this micrograph in relation to the AP boundary. Anterior En protein is not detectably altered by the absence of rasp^–. (J) A large posterior rasp^– clone is marked by the absence of GFP. This clone defines the position of the AP boundary from the posterior side. (K) The domain of anterior En protein is reduced in this disc (compare with H). (L) Overlay of J and K.

Return to article