Fig. 3. Lineage tracing of trunk shows ISVs derive from the LPM, via the axial vessels. (A) Fluorescein dye is uncaged by the laser in cells in the LPM of a nine somite embryo adjacent to somite nine. (B) Laser activation of cells in the LPM leads to labeling of aortic, connector and T-cells. (C) Cross section of a labeled ISV. (D) Cross section of ipsilateral and contralateral labeled DLAV cells. In A,B, anterior is towards the left. Dorsal is upwards. DLAV, dorsal longitudinal anastomotic vessel; ISV, intersegmental vessel; A, dorsal aorta; V, posterior cardinal vein; nt, neural tube; nc, notochord; s, somite.
