Fig. 3. The loss of Ptc activity affects the development of the eye-antennal disc. The discs were stained with Phalloidin and examined by confocal microscopy. (A,B) Control (ptc^H84/CyO or ptc^hdl/CyO) and ptc^hdl/ptc^H84 mutant discs, respectively, dissected from the late 3rd instar larval stages. (C,D) Control (ptc^H84/CyO or ptc^hdl/CyO) and ptc^hdl/ptc^H84 mutant discs, respectively, dissected from very late 3rd instar discs and cultured in vitro overnight (see Materials and Methods). The development of the discs in vitro appears to be equivalent to approximately 1-day old brown pupae.

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