Fig. 2. Inactivation of the Erbb2 gene. (A) At the NMJ of soleus muscle in wild-type mice Erbb2 (red) formed co-clusters with AChR (green) as identified by -bungarotoxin staining (a-Btx). (B) No Erbb2 protein was detected at the NMJ of Erbb2-deficient mice. Diffuse staining of Erbb2 was seen in terminal Schwann cells. (C-E) Myoblasts from wild-type and mutant mice were isolated from P2 mice and induced to form myotubes in culture. Agrin was added to induce AChR clusters. In wild-type mice, Erbb2 (red) co-localized with AChR clusters (green) (C). In the mutants, agrin induced AChR clusters, but Erbb2 was absent from the clusters (D). Utrophin (red) still co-clustered with AChR (green) in the mutant muscle fibers (E). Scale bars: 15 µm in A,B; 10 µm in C-E.

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