Fig. 8. (A,B) DiI-labeling of trigeminal ganglia. Ophthalmic (V₁) and maxillomandibular (V₂) lobes of the trigeminal ganglion contain DiI-labeled cells only when DiI also labeled cells of the ectoderm. (B) Horizontal section through plane b shown in A shows labeled cells in the trigeminal ganglion (V₁,V₂) rostral to the otic vesicle. (C-F) Sox10 immunopositive cells excluded from cranial ganglia. (C) Lateral view of stage 24 embryo immunolabeled with Sox10 antibody. Immunopositive cells are seen in the rostral pharyngeal arches and dorsally surrounding V₁ and V₂ lobes of the trigeminal ganglion, the eye and the otic vesicle (ov). d and f indicate horizontal planes of section shown in D and F, respectively. e indicates plane of transverse section shown in E taken from a stage 24 companion embryo. Sox10 immunopositive cells are seen between the otic vesicle and V₂ of the trigeminal ganglion (arrow in D) and lie medial to, but are excluded from, the placode-derived ganglia V₁, V₂ and eye (ey) in E. (F) Immunopositive cells are found in the mandibular arch (arrow) and weakly throughout the mesenchyme of the caudal branchial arches. Scale bars: in B, 200 µm for A,B; in C, 200 µm for C-F.

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