Fig. 6. Altered expression patterns of unpg, en and Poxn in brains of otd^–/– embryos. Laser confocal microscopy of stage 13/14 embryos, reconstructions of optical sections. (A) Frontal view, (B,C) lateral views. (A,B) otd^JA101; P{3'lacZ}unpg^r37/+, (C) otd^JA101. The extend of optical sections used to reconstruct the frontal view in A is the same as in Fig. 3B. Broken rectangles in the summary schemes indicate the region of the embryonic brain shown at higher magnification in B,C. (A,B) Triple-immunolabelling with anti-HRP (red), anti-ß-gal (green, yellow) and anti-EN (blue, white). Arrows indicate the anterior shift of brain-specific unpg expression into the anterior deutocerebrum (compare with Fig. 4D,E). Note that the protocerebrum is missing in otd mutants (Hirth et al., 1995), resulting in the absence of en-b1-expressing cells, whereas en-b2-expressing cells delimiting the deuto-tritocerebral boundary are present (A,B, arrowheads). (C) Double-immunolabelling with anti-HRP (red) and anti-POXN (green, yellow). Arrow indicates the normal position of the posterior POXN stripe in the posterior deutocerebrum, which is absent in the otd-null mutant embryo. PC, protocerebrum; DC, deutocerebrum; TC, tritocerebrum; Sub, sub-oesophageal ganglion; VNC, ventral nerve cord.

Return to article