Fig. 5. dpp is capable of stimulating PGC proliferation during early ovarian development. (A,B) A C587-gal4/UAS-GFP gonad labeled for GFP (green) and Hts (red), showing the expression pattern of a target gene controlled by the GAL4 line in the majority of the somatic cells. (B) The boxed area in A, showing that GFP is only expressed in most of the somatic cells (arrowhead) but not in the PGCs (outlined) that are identified by the presence of spectrosomes (arrow). (C,D) A C587-gal4/UAS-dpp; Dad-lacZ/+ gonad labeled for lacZ (red) and Hts (green), showing that overexpressed dpp induces expression of Dad-lacZ in all the PGCs as well as somatic cells. (D) The boxed area in C, showing that all the PGCs (some PGCs not adjacent to TFs/cap cells are outlined) and somatic cells (arrowhead) express Dad. (E) An early pupal wild-type gonad labeled for dead cells by ApoTag (green, nucleus) and Vasa (red, cytoplasm), showing a dying germ cell (arrow) and dying somatic cells in the anterior (arrowhead). (F) A C587-gal4/UAS-dpp gonad at the early pupal stage labeled for BrdU (green, nucleus) and Vasa (red, cytoplasm), showing that in the dpp-overexpressing gonad BrdU no longer preferentially labels anterior PGCs (arrowhead) and also labels more posterior PGCs (arrows) than in the control. Scale bars: 20 µm for A,C,E,F.

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