Fig. 7. Vulval morphogenesis defects caused by lin-11 RNAi. (A,B) Wild-type. (C-E) Early Pn.pxxx stage heat shocked hs-dslin-11i animals. (A) During mid-L4 stage, the vulD nuclei are located in the same plane of focus (arrows). (B) Same animal as in A when viewed at different focal plane. Thick arrows mark the 1° lineage cell nuclei, vulE and vulF–all seen together. (C) A hs-dslin-11i animal having defects in the positioning of vulval nuclei. In this focal plane, only the P5.p lineage presumptive vulD nucleus is visible. (D) The same animal as in C when viewed at different focal plane. The presumptive vulD nucleus of the P7.p lineage (thin arrow) is seen along with the vulF nuclei (thick arrows). vulE pair is not visible in this plane. (E) A weak Pvul phenotype seen in some hs-dslin-11i adults. Anterior is towards the left. Scale bars: in A, 8 µm for A-D; in E, 10 µm.

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