Fig. 6. Ectopic Xlim5-expressing cells express a marker of mature somites. Embryos injected with either ß-gal RNA alone (A) or ß-gal + Xlim5 RNA (B) vegetally at the 32-cell stage were fixed at the tailbud stage and immunostained after cryosectioning. Sections were stained with mAb 12/101 (somite, red) along with an antibody against ß-galactosidase (green). Embryos injected with ß-gal alone show non-overlapping staining of 12/101 with ß-gal (arrowhead) while a population of Xlim5-co-injected cells shows colocalization of the two antibodies (arrow, yellow). The slight green staining in the epidermis is due to background staining.

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