Fig. 5. Induced ubiquitous expression of sema3a1 induces constriction of the dorsal aorta and blocks flow of blood cells in the trunk vascular system. In all panels, embryos are 30 hpf. Blood cells flow through the dorsal aorta (right-pointing arrow) and axial vein (left-pointing arrow) in wild-type embryos (A) but not in hsp70:^gfpsema3a1^myc embryos following heat induction at 15 hpf (B,C). Asterisk in C denotes abnormal cavity filled with putative blood cells. When labeled with Berlin Blue ink, the dorsal aorta can be seen to be constricted in hsp70:^gfpsema3a1^myc embryos following heat induction at 15 hpf (arrow in E) compared with wild-type embryos (D). In yot embryos, the lumen of the dorsal aorta was tapered (arrow in F). High pressures that caused the cardinal vein to rupture (asterisks in F) were required to fill the dorsal aorta with ink. Scale bar: 20 µm.

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