DEV00811 Supplemental Data

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. (A) Alignment of amphioxus hairy proteins with selected vertebrate and invertebrate hairy proteins conducted by ClustalW. The human and murine HES2 sequences are added to show their similarity to the Fugu her10 genes. c, chicken; Dm, Drosophila melanogaster; Fr, Fugu rubripes; h, human; m, mouse; Tc, Tribolium castaneum; X, Xenopus laevis; zf, zebrafish. (B) Percentage similarity of the BHLH domain of amphioxus Hairy genes A to G to representative hairy and HES2 genes of vertebrates and invertebrates.

• Supplemental Table 1
• Supplemental Table 2
• Supplemental Figure 2 -

  Fig. S2. Neighbour-joining phylogenetic tree relating the amphioxus Hairy proteins with the human, mouse, zebrafish, Drosophila and Fugu Hairy and Enhancer of Split proteins. The tree has been rooted using the human and the mouse HERP sequences (Hey-class). The numbers refer to the bootstrap values. Note that Fugu Her10 genes group with the enhancer of split group HES2 (red shading), whereas the rest of invertebrate and invertebrate hairy genes group together (blue shading) Species abbreviations are: Ce; C.elegans; Dm, Drosophila melanogaster; Fr, Fugu rubripes; h, human; m, mouse; Tc, Tribolium castaneum; zf, zebrafish.