Fig. 1. The bowl and odd genes give similar phenotypes to Notch when mutated but are expressed at only a subset of Notch-dependent boundaries. (A,B) Organization of segments in wild-type prothoracic leg. The femur, tibia, tarsomeres (t1-t5), pretarsus (pt) and sex comb (sc) are indicated. (C) Notch mutations result in fusion and truncation of tarsomeres (N^ts/Y prothoracic leg, t1-t5 segments are fused). (D-H) Legs containing clones of cells mutant for bowl (bowl²; D-F) or odd (odd⁵; G-H) result in similar defects to Notch. (D,G) Melanotic tissue at femur/tibia joint (arrow) and (D,D') fused tibia/t1 (arrowhead; inset D' shows higher magnification), reduced t1 and aberrant sex-combs. (E) Severe fusion and truncation of tarsus when bowl clones occupy most of the distal leg. (F,H) Single bowl (F) or odd (H) clone affecting t1-t4 in prothoracic leg (marked with forked, similar results were obtained with yellow), causing partial fusions in t2-t5 (arrowheads) and associated truncation. (I-I'') Bowl (magenta) is expressed with E(spl)mß-lacZ (green) at the t5/pretarsus boundary (arrowhead) but not at other sites within the tarsus. (J-K'') Bowl protein (magenta) and odd-lacZ expression (green) in everting pupal (J-J'') and third instar (K-K'') leg discs. Expression occurs at intersegment/joint boundaries (tibia/t1, arrows; t5/pretarsus, arrowheads), where the two genes are coexpressed (J',K'; regions of overlap appear white). Expression of Bowl appears slightly broader than odd-lacZ.

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