Fig. 4. bowl is required for normal patterning of tarsal segments. Expression of Bab2 (anti-Bab2, blue) and E(spl)mß-CD2 (anti-CD2, red) in tarsal region of a wild-type pupal leg disc (A) and in a leg disc containing bowl² mutant clone (B; mutant cells are marked by absence of GFP, green). Bab expression extends proximally in the mutant cells (arrowhead, B,B') and E(spl)mß-CD2 is disrupted (arrow, B,B''). Inset in B'' (boxed region in B) shows relationship between clone boundaries (green) and E(spl)mß-CD2 expression (red); mutant cells at the edge of the clone express E(spl)mß-CD2 at wild-type levels. (C,D) Expression of Bab2 (anti-Bab2, cyan) and Dac (red) in wild-type pupal leg disc (C) and in a bowl² clone crossing t1 (absence of GFP, arrows, D-D''). The bowl² clone results in ectopic Bab2 (blue, D,D') and decreased Dac (red, D,D''); levels appear higher near the distal edge of the clone because of the fold in the epithelium. (E,E') bowl¹ clones in proximal tarsus/tibia, Bab2 (blue) is elevated in t1 (arrowhead) and Bowl staining (red) is absent from mutant clones (*). (F-F'') bowl² clones (absence of GFP, arrows) in distal tarsus of late third-instar leg disc result in elevated Bab2 (blue) and reduced BarH1 (red). Elevated Bab2 in more proximal clone is also seen (arrowhead).

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