Fig. 5. (A,D,G) Expression of the eHAND (A), dHAND (D) and mouse Anf (G) genes in the normal heart. Both eHAND and mouse Anf are expressed in the left ventricle, whereas dHAND is expressed in the right. (J,M,R) These patterns of expression were also confirmed in tissue sections. (B,E,H) In the MHC-Tbx5 transgenic mice, both eHAND and mouse Anf were induced in the entire ventricle, whereas dHAND was repressed. (K,N,Q) This was also confirmed in tissue sections. Histological examination of these sections revealed that ventricular septum formation was disrupted with a tiny bulge at the right-most end (black arrowhead). (C,F,I) In the MLC-2v-Tbx5 transgenic mice, the right ventricle expanded (red arrowhead). In this region, both the eHAND and mouse Anf genes were induced, and the dHAND gene was repressed. (L,O,R) Histological analysis revealed the swelling of the right ventricle (red arrowhead in L). In this swelling, eHAND was induced in a gradient manner (red arrowhead in L) and was absent from a region near the septum. (O) In this small region, dHAND is expressed (red arrowhead), whereas it is repressed in the rest of right ventricle. (R) Mouse Anf was also induced in the right ventricle of this transgenic mouse.

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